The Biological NanoStructures Lab (BNL) is a 1600 square foot shared multi-user environment for biological synthesis and analysis. Genomics & molecular biology, recombinant protein expression, purification & analysis and tissue culture will be available on a recharge basis.
The Autoclave Lab is open to the campus community. At this time all instruments in the Autoclave Lab can be used at no charge, a recharge system is expected to be in place within the fall quarter 2009. Access requirements are: completed general lab safety class (EHS), participation in lab specific safety instructions and completed instrument specific training.
Lab specific safety instructions and instrument specific training will be held on the following two dates:
July 15, 3:00pm in CNSI 3208
August 5, 12:00pm in CNSI 3208
RSVP is appreciated.
| Equipment | Model | Specifications | Intended Use |
|---|---|---|---|
| Large Autoclave | Tuttnauer 69120 SP-1A | 690 L capacity steam sterilizer | Autoclave biohazard waste (BSL 1 and 2) |
| Small Autoclave | Tomy ES 315 | 50L capacity steam sterilizer | Autoclave media and clean labware |
| Labware Washer | Labconco Steamscrubber 4400430 | 150L capacity, DI rinse option | Cleaning of labware |
| Baking oven | Binder ED115 | 115L; 300°C max Temp | Bake labware for RNA experiments, Bake labware to decontaminate from bacteriophages, Dry sterilization |
Biohazard waste can be autoclaved by users or delivered in biohazard bags for autoclaving and disposal through CNSI. In both cases, only correctly labeled biohazard bags rated for 140°C are accepted. Double containment is required for transport. Autoclave validation and record keeping according to Medical Waste Management Act will be managed through CNSI.
The tissue culture lab will be the next unit to open to the campus community. Access requirements will be the same as for the Autoclave lab. The lab specific safety instructions and instrument specific training will be included in a training class which is currently held once per quarter (see below for description).
The 3-4 week hands on cell culture class (2 x 3 h per week) is taught at a 2:1 student:teacher ratio. It is primarily designed for users with no or little tissue culture background and covers all basic aspects of mammalian cell culture, such as aseptic technique, culturing cells, live count of cells, freezing and thawing of cells, quality control.
February 10 - March 6 2009
July 7 - July 27 2009 full
TBD (fall quarter) open for sign up. Please contact Lab Director
The Main Lab is being developed and will include the resources summarized below. Funding has been secured for instruments without the (pending) remark. If you would like to co-sponsor instruments with a (pending) sign, please contact the Lab Director.
Claudia Gottstein
Phone: (805) 893-6054
Email: gottstein@cnsi.ucsb.edu
Office: 3221 Elings Hall
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Last Modified 20-nov-09