This core provides Next Generation Sequencing (NGS) library prep and sequencing resources to on- and off-campus researchers in and near Santa Barbara, CA. NGS (also known as High Throughput Sequencing) experiments are conducted on the Illumina NextSeq 500 which uses the same widely accepted technology found in other popular Illumina sequencers such as the HiSeq and MiSeq.
We are the only Illumina NGS facility in Santa Barbara and surrounding areas. The BNL is also the only facility within the UC system with a NextSeq 500. The versatile NextSeq 500 was selected to be our first instrument as it is a flexible platform, accommodating a variety of sequencing experiments. RNA-Seq, DNA-Seq, ChIP-Seq or targeted sequencing are just some of the types of NGS runs that can be performed on the NextSeq 500.
NGS samples can be prepped and submitted in the following ways:
- Sequencing done by BNL staff while library prep is done by user in their lab
- Library prep done by user in the BNL and sequencing by BNL staff
- Library prep and sequencing done by BNL staff