BNL Sequencing Request Form

Please fill in this form as accurately as possible so that we can proceed with your sequencing.
Please contact Jen Smith at the BNL if you have any questions.

User
Accounting Information
Account Number Format = 8-######-#####-#
Principal investigator
Sample Details
DNA Insert + Adapters
Concentration in nM, using expected library size (DNA insert + adapters).
Which Run Kit Do You Want to Run?

NextSeq Run Type

and Output

75 Cycles 150 Cycles 150 Cycles 300 Cycles  
High Output Mid Output High Output Mid Output  
Number of Reads Up to 400 M  Up to 130 M  Up to 400 M  Up to 130 M  
Read Length
SR / PE
1 x 75 bp 
2 x 37 bp
1 x 150 bp 
2 x 75 bp
1 x 150 bp 
2 x 75 bp
1 x 300 bp 
2 x 150 bp
 
Yield ~30 Gbp ~19.5 Gbp ~60 Gbp  ~39 Gbp  
Price $1745 $1395 $3115 $2195  

 

 

MiSeq Run Type

and Output

 600 Cycles 

 150 Cycles     300 Cycles   500 Cycles   300 Cycles   300 Cycles 

 500 Cycles 

v3 v3   v2 v2 Micro Nano Nano
Number of Reads Up to 25 M  Up to 25 M    Up to 15 M Up to 15 M Up to 4 M  Up to 1 M  Up to 1 M
Read Length
SR / PE
1 x 600 bp 
2 x 300 bp
1 x 150 bp 
2 x 75 bp
  1 x 300 bp 
2 x 150 bp
1 x 500 bp 
2 x 250 bp
1 x 300 bp 
2 x 150 bp
1 x 300 bp 
2 x 150 bp
1 x 500 bp 
2 x 250 bp
Yield ~15 Gbp ~3.8 Gbp   ~4.5 Gbp  ~7.5 Gbp ~1.2 Gbp ~0.3 Gbp ~0.5 Gbp
Price $1800 $1215   $1360 $1500 $720 $560 $620
Please review the table carefully when choosing your preferred run. Contact Jen at jsmith@cnsi.ucsb.edu with questions.
Run Details
For example, a 150 cycle PE run will read 75 bp on each side of the DNA molecule (PE75).

Please select the day you plan to bring your sample library in for sequencing. You will be added to the sequencing queue once we receive your pooled, ready-to-run library.

Date Requested
You will be contacted by email to inform you of the actual date assigned. The assigned date will be determined once we receive your completed sample library and index list.
If you would like PhiX added to your run, please let us know the PhiX percentage in the box below.
If you have any special run instructions, please enter them in the Comments box below. If there are multiple account numbers you would like us to charge, enter the recharge account number and the percentage of the NGS Run to charge to this account.