Please fill in this form as accurately as possible so that we can proceed with your sequencing. Please contact Jen Smith at the BNL if you have any questions. User First Name * Last Name * Email * Accounting Information Department / Program * - Select -Chemical EngineeringEcology, Evolution, and Marine BiologyInstitute for Collaborative BiotechnologiesMarine Science InstituteMolecular, Cellular, and Developmental BiologyNeuroscience Research InstituteStem Cell Biology and EngineeringOther... Department / Program Other... Account Number * Account Number Format = 8-######-#####-# Project Code Principal investigator First Name * Last Name * Sample Details What kind of NGS run is this? * Whole Genome Sequencing RNA-Seq Amplicon Sequencing ChIP-Seq Exome Sequencing Other... What kind of NGS run is this? Other... How many samples will you have pooled in one run? What is the expected average size (bp) of your library? DNA Insert + Adapters What is the expected concentration (nM) of your library? Concentration in nM, using expected library size (DNA insert + adapters). Which Run Kit Do You Want to Run? NextSeq Run Type and Output 75 Cycles 150 Cycles 150 Cycles 300 Cycles High Output Mid Output High Output Mid Output Number of Reads Up to 400 M Up to 130 M Up to 400 M Up to 130 M Read Length SR / PE 1 x 75 bp 2 x 37 bp 1 x 150 bp 2 x 75 bp 1 x 150 bp 2 x 75 bp 1 x 300 bp 2 x 150 bp Yield ~30 Gbp ~19.5 Gbp ~60 Gbp ~39 Gbp UCSB Price $1790 $1400 $3200 $2200 MiSeq Run Type and Output 600 Cycles 300 Cycles 500 Cycles 300 Cycles 300 Cycles 500 Cycles v3 v2 v2 Micro Nano Nano Number of Reads Up to 25 M Up to 15 M Up to 15 M Up to 4 M Up to 1 M Up to 1 M Read Length SR / PE 1 x 600 bp 2 x 300 bp 1 x 300 bp 2 x 150 bp 1 x 500 bp 2 x 250 bp 1 x 300 bp 2 x 150 bp 1 x 300 bp 2 x 150 bp 1 x 500 bp 2 x 250 bp Yield ~15 Gbp ~4.5 Gbp ~7.5 Gbp ~1.2 Gbp ~0.3 Gbp ~0.5 Gbp UCSB Price $1940 $1400 $1540 $730 $570 $640 Run Kit * NextSeq 75 Cycles NextSeq 150 Cycles NextSeq 300 Cycles MiSeq 600 Cycles MiSeq 150 Cycles MiSeq 300 Cycles MiSeq 500 Cycles Please review the table carefully when choosing your preferred run. Contact Jen at jsmith@cnsi.ucsb.edu with questions. Run Kit Output * NextSeq Mid Output NextSeq High Output MiSeq v3 MiSeq v2 Standard MiSeq Micro MiSeq Nano Run Details Run Type * Single End (SE) Paired End (PE) For example, a 150 cycle PE run will read 75 bp on each side of the DNA molecule (PE75). BaseSpace Account * Your NGS run data will be transferred to you using the Illumina BaseSpace cloud server. If you have not already, set up an account on BaseSpace by going to https://basespace.illumina.com. Enter the email address associated with your BaseSpace account below. Please select the day you plan to bring your sample library in for sequencing. You will be added to the sequencing queue once we receive your pooled, ready-to-run library. Date Requested Date * Month MonthJanFeb Day Day12345678910111213141516171819202122232425262728293031 Year Year2021 You will be contacted by email to inform you of the actual date assigned. The assigned date will be determined once we receive your completed sample library and index list. Comments If you have any special run instructions, please enter them in the Comments box below. If there are multiple account numbers you would like us to charge, enter the recharge account number and the percentage of the NGS Run to charge to this account. PhiX If you would like PhiX added to your run, please let us know the PhiX percentage in the box below. Leave this field blank
